Induced Spawning Activity of African Catfish (Clarias gariepinus, BURCHELL 1822) Using Different Fish Pituitary Gland

Fabio C. Ruaza Jr.


A total of 27 Clarias gariepinus breeders (18 males and 9 females) with the female body weight ranging from 750g to 2100g were used to observe the spawning activity of the fish. The study was conducted to determine the reproductive variables of C. gariepinus, such as gonadosomatic index, fecundity, fertility, and hatchability. The pituitary glands (PG) collected from C. gariepinus, Cyprinus carpio, and Oreochromis niloticus were used for induction. The spawning fecundity of C. gariepinus injected with C. gariepinus pituitary gland extract oscillated at 79, 510 eggs, while the recipient of C. carpio pituitary gland extract yielded 106, 550. However, the fish administered with O. niloticus pituitary gland extract did not respond to the induction since there was no egg yield during the stripping procedure. The gonadosomatic index of C.gariepinus and C.carpio induced fish were 12% and 12.23%, respectively. In terms of fertilization rate, the C. gariepinus (88.4%) is higher than C. carpio (76.6%). Concerning the hatchability rate, it was observed that after 31 hours of incubation, the eggs were hatched with a rate of 58.6% and 67.6% for the C. gariepinus and C. carpio induced fish, respectively. Statistical analysis shows no significant difference both in hatchability rate and fertility rate, which implies that both C. gariepinus and C. carpio PG could be used as effective inducing agents in the spawning activity of African Catfish, C. gariepinus.


Clarias gariepinus; induced spawning; pituitary gland; reproductive variables

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Adebayo, O.T. (2006). Reproductive performance of African Clariid Catfish Clarias gariepinus broodstock on varying maternal stress. Journal of Fisheries International, 1(1-2): 17-20 doi: jfish.2006.17.20

Adebayo, O.T & Fagbenro, O.A. (2004). Induced ovulation and spawning spawning of pond raised African Giant Catfish, Heterobranchus bidorsalis by exogenous hormones. Aquaculture, 242: 229-236.

Agbebi, O.T, Faseluka, O., & Idowu, A.A. (2013). Effects of Various Latency Periods on the Fertilization, Hatchability and Survival of Clarias gariepinus. Journal of Fisheries and Aquatic Science, 8: 178-183.

Agropedia. (2012). Induced Breeding with Pituitary Gland Extraction from Fish. Generic crop Karnataka Northeast Dry India UAS-Raichur.

Alok, D., T. Krishnan, G.P. Talwar, & L.C. Garg. (1993). Induced spawning of Catfish, Heteropneustesfossilis (Bloch), using D-Lys6 Salmon gonadotropin-releasing hormone analog. Aquaculture 115(1-2):159-167.

Ayinla, O.A. (2003). Integrated fish farming: A veritable tool for poverty alleviation/hunger eradication in the Niger Delta Region. Proceedings of the Fisheries Society of Nigeria, Owerri, Nigeria (Eyo, A.A and Atandaeds.):40-41.

Boyd, C.E. (1979).Water quality in warm water fish ponds. Auburn University Agricultural Experimental Station, Auburn, Alabama: 358

Britz, PJ & Hecht, T.. (1988). Artificial propagation and fry production. In: Hecht, UysTW and Britz PJ (Ed). The Culture of Sharptooth catfish Clariasgariepinusin South Africa. South African National scientific programmes Report No. 153. FRD/CSIR, Pretoria; 36-61.

Dada, A.A and Ebhodaghe, B.E. (2011). Effects of Garcinia kola seed meal on Egg Quality of the African Catfish (Clariasgariepinus) (Burchell) Broodstock. Cameron Journal of Experimental Biology vol. 7 no. 1, 1-8.

De Graaf, G., Galemoni, F., & Banzoussi, B. (1995). The artificial reproduction and fingerling production of the African catfis Clariasgariepinus (Burchell, 1822) in protected and unprotected ponds Aquaculture Research 26: 233-242.

De Graaf, G. & Janssen, H.. (1996). Artificial Reproduction and Pond Rearing of the African catfish Clariasgariepinus in Sub-Saharan Africa- A Handbook, Vol. 362. FAO Fisheries Technical Paper, Rome 73pp.

Dekempe P. & Micha JC. (1994). First guidelines for the culture of Clariaslazer in Africa. Aquaculture; 4:227-48.

Donnelly, B.G. (1973). Aspects of behavior in the catfish Clariasgariepinus (Pisces: Clariidae) during periods of habitat desiccation. Arnoldia 6:1-8.

FAO. (1990). Utilization of tropical food and animal product. FAO food nutrition. Paper 47 FAO Rome pp8-21

Gadissa, S. & L.P. Devi. (2013). Evaluation of Spawning Induction of African Catfish(Clariasgariepinus) by Heteroplastic Hypophysation. International Journal of Fisheries and Aquatic Studies 1(1):22-25.

Haniffa, M.A.K. & S. Sridhar. (2002). Induced Spawning of Spotted Murrel (Channa punctatus) and catfish (Heteropneustesfossilis) using human chorionic gonadotropin and synthetic hormone (Ovaprim) Veterinarski Archiv 72:51-56.

Hecht, T., Uys,W., & Britz, PJ. (1988). The culture of the sharp toothcatfish in South Africa. South African National Scientific Programmes Report 1988; 153.

Hecht, T., Saaymen, JE., & Poolling, L. (1982). Further observations on induced spawning of sharp tooth catfish, Claraisgariepinus(Claridae: Pisces). Water S A 1982;8(1):101-107.

Hogendoorn, H. & Vismans, MM. (1980). Controlled propagation of African catfish Clarias lazera(Cuvier &Valenciences). II Artificial reproduction. Aquaculture 1980; 21:39.

Hogendoorn, H. (1983). The African catfish, Clarias lazera (Cuvier &Valenciennes, 1840) A new species for aquaculture (Doctoral dissertation). Agriculture University, Wageningen the Netherlands.

Hossain, B. et al. (2012). Comparative Study of Carp Pituitary Gland (PGE) Extract and Synthetic Hormone Ovaprim Used in the Induced Breeding of Stinging Catfish, Heteropneustesfossilis (Siluriformes: Heteropneustidae). Our Nature 10:89-95.

Howaida, R.G., Hanlon, T.H., Mahmoud, H.H., & Eletreby, S.G. (1998). Maturation, fecundity, seasonality and reproduction of two commercially valuable cuttlefish, Sephiapharaonis and S. dollfusi in Suez Canal. Journal of Fisheries Research, 36(23): 99-115.

Kohil, M.P.S & U.C. Goswani. 1987. Spawning Behavior of a Freshwater Air breathing Indian Catfish Heteropneutesfossili(Bloch).Matsya12:180183.

Legendre, M.J., Slembrouck, J., Subagja, J., & Kristanto, A.(2000). Ovulation Rate, Latency period and Ova Viability after GnRH- or- hCG- induced breeding in the Asian catfish Pangasiushypophthalmus (Siluriformes, pangasiidae) Aquatic Living Resources, 13: 145-151.

Macfarlane, C., Drew, P., Hoysak, J., Robin Liley, N. , Matthew, J., & Gage. G. (2009). In vitro fertilization experiments using sockeye salmon reveal that bigger eggs are more fertilizable under sperm limitation. DOI: 10.1098/rspb.2009.0295. Retrieved from

Mollah, MFA., Taslima, K., Rashid, H., Hossain, Z., Sarowar, MR., & Khan, MRK.. (2011). Embryonic and larval development of critically endangered riverine catfish Rita rita. Eurasia J Biosci 5: 110-118. DOI: 10.5053/ejobios.2011.5.0.13

Musa, A.S.M & Bhuiyan, A. (2007). Fecundity on Mystusbleekeri (Day, 1877 ) from the River Padma Near Rajshahi City. Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh.

Nayak, P.K., Mishra, TK., Singh, B.N., Pandey, A.K. & Das, R.C. (2001). Induced Maturation and Ovulation in Heteropneutesfossilis by using Ovaprim for Production of Quality eggs and larvae. Indian Journal Fisheries 48(3):269-275.

Nchedo, A.C. & Chijioke, O.G. (2012). Effect of pH on Hatching Success and Larval Survival of African Catfish (Clarias gariepinus). Nat Sci, 10(8):47-52(ISSN:1545-0740).

Ndimele, P.E & Owodeinde, F.G. (2012). Comparative Reproductive and Growth Performance of Clariasgariepinus (Burchell, 1822) and Its Hybrid Induced with Synthetic Hormone and Pituitary Gland of Clarias gariepinus. Turkish Journal of Fisheries and Aquatic Sciences 12: 619-626. DOI: 10.4194/1303-2712-v12_3_09.

Olumuji, O.K. and Mustapha, M.K. (2012). Induced Breeding of African Mud, Clarias gariepinus(Burchell 1822), Using Different Doses of Normal Saline Diluted Ovaprim. J. Aquacult Res Dev 3:133 DOI:10.4172/2155-9546.1000133.

Oyelese, O,A. (2006). Water Temperature a Determinant of Fertilization and Hatchability Rates in Artificial Breeding of Clariasgariepinus (Teleostei: Clariidae). Department of Wildlife and Fisheries Management, University of Ibadan, Ibadan, Nigeria Research Journal of Biological Sciences 1(1-4): 83-87.

Phelps, R.P.; R. Hastey; A. Tendetar; L. Linley; N. Papanikos; R.A. Dunham (2007). Effects of Temperature on the Induced Spawning of ChannelCatfish and the Production of Channel x Blue Catfish Hybrid Fry. Aquaculture, 273:80-86.

Richter, CJJ. (1976). Arfican catfish, Clariaslazera (C. & V.), A new possibility for fish culture in tropical regions in Masc PapLand bouwhogeshWageningen; 13:51-71.

Sahoo, S.K. Giri, S.S., Chandra, S., & Mohapatra, B.C. (2008). Evaluation of Breeding Performance of Asian Catfish Clariasbatrachus at Different Dose of HCG and Latency Period Combinations. Turkish Journal of Fisheries and Aquatic Sciences 8:249-251.

Sydenham, B.H.J. (1980). New species of Clarias for West Africa. Revue de Zoologieafricaine, 94(3):659-677.

Thakur, NK & P. Das. (1986). Synopsis of biological data on magur Clarias batrachus (Linnaeus, 1758). Central Inland Fisheries Research Institute Barrackpore 1986, 41:81

Van der Waal, BCW. (1974). Observations on the breeding habits of Clariasgariepinus (Burchell). Journal of Fish Biology, 6:23-27.

Vitule, J.R.S., Umbria, S.C., & Aranha, J.M.R.. (2006). Introduction of the African catfish Clariasgariepinus (BURCHELL 1822) into Southern Brazil. Biological Invasion, 8:677-681.

Winemiller, K.O & Kelso-Winemiller, L.C. (1996). Comparative ecology of catfishes of the upper Zambezi River floodplain. Journal of Fish Biology, 49:1043-1061.

Wooton, R. J. (1990). Ecology of Teleost Fishes Istedtn. Chapman and Hall, London. 344pp.

Yalcin, S., Akyurt, I., & Solak, K. (2001). Certain reproductive characteristics of the catfish (Clariasgariepinus Burchell, 1822) living in the River Asi, (Turkey). Turkish Journal of Zoology, 25:453-460.

Yusuf, K., Dada, S.A., & Abari, M.A. (2013). Length Weight Relationship, Fecundity and Gonadal Development of The African Catfish (Clariasgariepinus) From Doma Dam, Nasarawa State, Nigeria. Department of Fisheries Technology, College of Agriculture Lafia, Nasarawa State, Nigeria.


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